Plasma Replacement Procedure:
The lipemic specimen is initially run through the automated instrument and the results recorded.
- A specimen of blood is aliquoted into a tube.
- The height of the sample in the tube is marked by a line.
- The sample is then washed with instrument diluent followed by
centrifugation and supernatant removal until the supernatant is clear.
- An amount of diluent is added to bring the sample to the marked line. This needs to be done as accurately as possible.
- The washed sample is then run through the analyzer.
Correction factor = (RBC count on untreated specimen) / (RBC count on washed specimen)
Hemoglobin to report = (hemoglobin of washed specimen) * (correction factor)
Performing a Complete Blood Count (CBC) on a Lipemic Sample
Source: MELINA+ Algorithms